To demonstrate the suitability of PSMA-I&F as a targeted probe for the intraoperative detection and fluorescence-guided resection of PSMA-expressing prostate carcinoma tissue, and to investigate the general compatibility of a Cy5-conjugated fluorescent probe with clinically used fluorescence cameras such as the Firefly laparoscope, proof-of-concept intraoperative and ex vivo fluorescence imaging using PSMA-I&F was performed in an LNCaP tumor–bearing mouse (Fig. 6). The gene discussed is FOLH1; the disease is prostate carcinoma.