To reveal the infection status of G. duodenalis in farmed bamboo rats, the prevalence and assemblages of this parasite in bamboo rats from six farms in four cities of Hunan Province, China, were determined, and the genetic diversity of G. duodenalis was also investigated by using the multilocus genotyping technique based on three gene loci [46–48], namely beta giardin (bg), triosephosphate isomerase (tpi) and glutamate dehydrogenase (gdh). This evidence concerns the gene TPI1 and infection.