HIRA and infection: To analyze the requirement of the histone H3.3 chaperones for the formation of the vDCP NBs and HSV-1 chromatinization, DAXX, ATRX, HIRA or UBN1 were depleted by shRNAs in normal BJ cells or cells constitutively expressing e-H3.3 prior to infection with in1374 and completion of the experiments.