Immunoblotting technique was performed along with IgE detection by means of previously cited tests in 4 studies on general asymptomatic population [30, 14, 48, 49]; 4 studies on symptomatic population with allergies to any kind of allergen [14, 30, 31,50]; 1 study on patients presenting to hospital for controls [51] and 2 studies on patients with digestive system disorders [32, 49]. This evidence concerns the gene IGHE and allergic disease.