IGHE and anisakiasis: Successively, another variation of rAni s1 and rAni s7 indirect ELISA was introduced by Anadon et al. [36] representing the most specific serum test to diagnose anisakiasis, revealing IgE in 40.2% out of 493 allergic subjects in Madrid, with respect to 52.7% positivity prevalence measured by ImmunoCAP from the same serum samples.