Chemotherapy enhances the immune activity or the reversal of immunosuppression.7 18 Some studies revealed that changes in the levels of CD8+ or FOXP3+ TILs induced by chemotherapy can be used as a prognostic marker in aggressive breast cancer subtypes, such as TNBC.15 19 20 However, the predictive value of changes in lymphocytic subpopulations after NAC in all breast cancer subtypes has not been discussed sufficiently. The gene discussed is CD8A; the disease is breast cancer.