In contrast to fatty liver sham operation group, there was a marginally (TNF-α and IL-6) or significantly (TLR-4 and Nlrp3) higher level of expression of all of the above factors by evaluating mRNA in the steatotic livers following 3 hr reperfusion after 15 min ischemia (Fig. 2). This evidence concerns the gene TNF and Hepatic steatosis.