Given the importance of this for our understanding of AD pathology, we confirmed binding between endogenous APP and LRP6 or Vangl2 proteins in rat primary cortical neuronal cultures by co-immunoprecipitation (Fig. 1c), confirming the data previously obtained by exogenous overexpression of the proteins in HEK293 cells9,20. The gene discussed is VANGL2; the disease is Alzheimer disease.