The KEGG pathway analysis showed that the significant pathways were transcriptional misregulation in cancer, FoxO signaling pathway, estrogen signaling pathway, PI3K-Akt signaling pathway, alcoholism, MAPK signaling pathway, adherens junction, Chagas disease (American trypanosomiasis), proteoglycans in cancer, focal adhesion, ErbB signaling pathway, ubiquitin-mediated proteolysis, B-cell receptor signaling pathway, toxoplasmosis, TNF signaling pathway, apoptosis, endometrial cancer, HIF-1 signaling pathway, Toll-like receptor signaling pathway, and AMPK signaling pathway. The gene discussed is AKT1; the disease is alcohol dependence.