While liver steatosis, a reduction of PGC-1α, a reduction of complexes of the respiratory chain as well as signs of oxidative stress were already present after 19 days of feeding, no increase in inflammatory markers was detectable, indicating that mitochondrial damage and oxidative stress precede the development of inflammation (Figure 7, Figure S4 in Supplementary Material). Here, PPARGC1A is linked to fatty liver disease.