IFNG and pulmonary tuberculosis: Experiments were carried out on peripheral blood mononuclear cells (PBMCs) isolated from the following groups of subjects: IFNγ release assay (IGRA) negative (IGRA-ve); IGRA positive (+ve); sputum positive, untreated pulmonary tuberculosis (PTB) and PTB subjects who have completed 6 months of anti-tubercular treatment (ATT) (Clinical summary in Table 1 and details in S1 Table).