We screened the 4 TFs in the TRRUST version 2 database query to get each TF target genes and the DEGs from four profile datasets in GEO and TF-target genes for intersection analysis to obtain 42 differentially expressed target genes of the TFs (key genes) (Table 6), which laid the foundation for our next step to construct the gene signaling regulatory network in HCC. This evidence concerns the gene TF and hepatocellular carcinoma.