Western blot analysis for Hmga1 and Hmga2 on sorted (lineagenegative, DAPInegative, Tomatopositive) cancer cells isolated from pancreatic tumours from KPHetCT;Hmga2+/+ and KPHetCT;Hmga2CK/CK mice confirmed the absence of Hmga2, the presence of the GFP fusion protein in Hmga2-deficient tumours, and unaltered Hmga1 expression (Fig. 4b). This evidence concerns the gene HMGA1 and cancer.