It was surprising that frontal cortex microglia CM from another late-stage AD case, AD2, did not show significant seeding, but upon further examination of Tau1-labeled neuropathology slides, the superior frontal gyrus from the AD2 case contained very little tau pathology characterized by flame-like interneuronal neurofibrillary tangles (Fig. 5b) compared to cases where microglia CM did induce seeding (Fig. 5c–e) and was more similar to causes with minimal tau pathology (Fig. 5f–h). This evidence concerns the gene MAPT and Alzheimer disease.