In an effort to highlight the functional role of the NOS2/NO system in glioma biology, the purpose of the current study was to explore the effect of the NOS2 inhibitor 1400W at no cytotoxic concentrations toward the proliferation and migration rate, clonogenic potential, and neurosphere generation ability of glioma cells expressing high levels of NOS2. The gene discussed is NOS2; the disease is glioma.