The high expression level of miR-224 connected with poor disease-free survival rate partially through suppression of SMAD4 that function as transmitters of TGF-β signals, and decreases the chemoradiosensitivity (CRT) in CRC, highlighting the function of miR-224 in the process of potential predictive marker for relapse following radical surgery of colorectal cancer [54]. The gene discussed is SMAD4; the disease is colorectal carcinoma.