Western blot was carried out as described previously [8] to study the expression of proteins - RPA1 and HSPH1, across HNSCC cell lines (CAL 27, FaDu and JHU-O28) compared to human normal oral keratinocyte (OKF6/TERT1) (Fig. 1B). The gene discussed is RPA1; the disease is head and neck squamous cell carcinoma.