SHBG and metabolic bone disorder: The serum E2 level of OVX group was lower than that of sham group, and the serum SHBG level displayed the contrary tendency (Figures 3(a) and 3(b)); the serum OPG, OPG/RANKL, and BALP of OVX group was lower than those of sham group, and the serum RANKL showed the contrary tendency (Figures 3(c)–3(f)), which provided other evidence of our successful establishment of the estrogen deficiency mediated bone metabolism disorder in the ovariectomized rat model.