For in vivo efficacy studies, two pediatric tumors, glial tumor D456 with lower nectin-1 expression and embryonal tumor X21415 with higher nectin-1 expression, and two adult tumors, UAB1066 with lower nectin-1 expression and GBM-12 with higher nectin-1 expression were tested. The gene discussed is NECTIN1; the disease is glioblastoma.