In this study, we examined archived tumor specimens obtained from a large cohort of patients with stage IB-IV GC for IHC expression of PD-L1 and CD8 in three distinct subtypes of GC (EBV+/MSI-H/EBV− and MSS) by QIA, and correlated them with mRNA expression level and manual interpretation. The gene discussed is CD274; the disease is neoplasm.