EEF1A1 and breast carcinoma: However, we find that the CpG methylation status measured for all CpG probes that were located in the 1000 base pairs upstream of the EEF1A1 transcription start site were very low in both normal breast tissue and breast carcinoma tissue and these minimal differences did not significantly change (Fig. 3B), indicating that low EEF1A1 mRNA expression is not caused by hypermethylation of the EEF1A1 promoter.