However, while Stat3 mRNA and STAT3 protein levels were similar in all genotypes, its steady state activation in HCC livers as revealed by pSTAT3 western blot and pSTAT3 immunohistochemistry remained largely unaltered even in D-KO mice with high variabilities (Supplementary Fig. 4A, B, C). The gene discussed is STAT3; the disease is hepatocellular carcinoma.