To address the effect of additional hepatic LEPR ablation in whole body IL-6Rα-deficient mice on HCC development, we subjected cohorts of Ctrl, LepRL−KO, IL-6RαKO and D-KO mice to the DEN-induced HCC model according to a protocol depicted in Figure 2J, which allows for metabolic as well as oncogenic characterization. Here, LEPR is linked to hepatocellular carcinoma.