The combination of all 6 proteins: AFP, B2M, GP73, GDF15, IGFBP2 and OPN displayed the best results in the 4 models tested in both the training and testing set in distinguishing HCC patients from healthy controls, with the highest accuracy rate of 0.923 (Figure 8D). The gene discussed is IGFBP2; the disease is hepatocellular carcinoma.