The average expression of AFP and GP73 in 132 HCC serum samples showed a dramatic increase (5.16 and 2.12-fold change) compared with the levels in healthy controls (mean concentration of 4.904 vs. 0.9404 μg/ml and 4.644 vs. 2.192 μg/ml, respectively). This evidence concerns the gene GOLM1 and hepatocellular carcinoma.