Treatment with TLR2 and TLR9 agonists resulted in similar induction of Tregs in non-TB uveitis, non-uveitis and non-uveitis TB groups, highlighting that lower induction of Tregs is specific to IOTB subjects and that induction of Tregs following direct ligation of TLR2 and TLR9 in CD4+ T cells was compromised in IOTB. The gene discussed is CD4; the disease is uveitis.