Western blot analysis to confirm the identity of the recombinant protein using anti-dengue antibody revealed that the CWPs of cells harboring pYEG-R-Co1-scEDIII-AGA-TER showed a band corresponding to the size of the fusion protein of Co1-scEDIII-AGA with the expected size of ~ 50 kDa (Fig. 3d), whereas no corresponding band was found in the culture filtrate of the transformant cells or in the cell fractions of Mock cells or wild-type cells, confirming the anchorage of the target protein Co1-scEDIII-AGA to the yeast cell wall. Here, AGA is linked to dengue disease.