Both enzymes were acetylated by PCAF.37, 131 Using immunoprecipitation of endogenous proteins and in vitro pull‐down assay with recombinant, a study identified and validated that PCAF as the acetyltransferase acetylating PGK1 K323 acetylation in liver cancer, this modification is characterized as an important positive regulation of PGK1 enzymatic activity and its oncogenic function.37 In addition, they further identified and validated that SIRT7 is the deacetylase of PGK1 at K323. The gene discussed is KAT2B; the disease is liver cancer.