IL‐10 is an anti‐inflammatory cytokine that has been demonstrated to directly improve both cisplatin‐ and ischaemia/reperfusion‐induced AKI.27 We previously observed that pFUS alone stimulated AKI kidneys to increase the production of interferon (IFN)γ.17 The potential involvement of IFNγ was intriguing, as culturing MSCs with pro‐inflammatory cytokines “primes” MSCs to produce more anti‐inflammatory factors and become a more effective therapeutic cell factory.28, 29 We initially performed a simple in vitro preconditioning study of MSC by culturing them with recombinant IFNγ. This evidence concerns the gene IFNG and acute kidney injury.