In this analysis the cis-vaccenic acid content positively correlated with GDM patients BMI (R = -0.39; p = 0.0442), and age (R = 0.56; p = 0.0012) and in NGT group vaccenic acid correlated negatively with FPG (R = -0.7, p = 0.0358), insulin (R = -0.72; p = 0.0298) and HOMA-IR (R = -0.67; p = 0.0499), and positively with QUICKI (R = 0.67; p = 0.0499), while stearic acid correlated negatively with FPG (R = -0.42; p = 0.03) and positively with LDL levels (R = 0.38; p = 0.04) in GDM group. This evidence concerns the gene INS and gestational diabetes.