GPC3 and malignant colon neoplasm: From the results of biomarkers assay, we found that no matter the HCS1220 cell or implantation tumor tissue, they all expressed the colon cancer biomarker CK20, CDX2, a-fetoprotein, hep-1, glypican-3 stained negative, the results demonstrated that the HCS1220 cell line originating from the intestinal tissue.