Since our PI polyamides recognize five nucleotides corresponding to the EVI1-binding site and three adjacent nucleotides at the 5′ or 3′ side of the binding site within the GPR56 promoter, both PIP/56-1 and PIP/56-2 did not affect expression of other EVI1-targeted genes tested in EVI1high AML cells, although we cannot completely exclude the possibility that GPR56-PIP treatment may influence expression of other EVI1-target genes besides GPR56. This evidence concerns the gene RUNX1 and acute myeloid leukemia.