Here in our research, a PLSR model was used to correlate the chromatographic data of ZZX and their pharmacodynamics indices, including the behavioral anti-anxiety indicators, contents of CRF, ACTH, CORT in serum, and the contents of monoamines and metabolites (NE, DA, DOPAC, HVA, 5-HT, 5-HIAA) in the brain. This evidence concerns the gene CRH and Anxiety.