ACTA1 and Hepatic fibrosis: At the chosen time-point (week 17), we observed a clear liver fibrosis in both DEN doses in male mice (DEN10/CCl4 and DEN50/CCl4), characterized by increased collagen accumulation (Sirius red), HSCs activation (α-SMA), increased population of CD68+ macrophages and activation of COX-2/IL-6 axis.