IRF8 and cancer: It is clear that the p-values are not statistically significant for limma, i.e., the p-value is 0.872 for IRF8, 0.959 for CECR1, and 0.867 for IL10RA. The middle panel shows the scatter plot of expression profiles versus InfiniumPurify purities, in which the correlations are all very high, especially, −0.68 for IL10RA. The high correlation indicates that the large within group variance of cancer samples is mostly caused by variation in purities for different samples, which dilutes the signals of DEGs.