The RP2D of the combination vaccine was 7.5 × 106 irradiated H1944 adenocarcinoma cells expressing CCL21 (multiplicity of infection of 500), 7.5 × 106 irradiated H2122 adenocarcinoma cells, and 15 × 106 GM.CD40L cells (1.1 mL). Here, CD40LG is linked to adenocarcinoma.