Moreover, DTS of a 9-gene set recurrently mutated in CLL (TP53, SF3B1, NOTCH1, NFKBIE, BIRC3, POT1, MYD88, XPO1, and EGR2) revealed 7 mutations which were not detected by WES due to their low Variant Allele Frequency (VAF) (Supplementary Table S3). This evidence concerns the gene BIRC3 and B-cell chronic lymphocytic leukemia.