Cognate pMHC on the GUV surface was required to recruit ZAP70 to the membrane, as indicated by the observation that pMHC comprised of a peptide not recognised by the 1G4 TCR (derived from the melanoma antigen gp100) produced no increase in mNeonGreen–ZAP70 fluorescence (Fig. S4). This evidence concerns the gene ZAP70 and melanoma.