To determine the expression and localization of LGR5 in glioma cells, LGR5 staining was performed in 3 types of glioma cell lines (U251, U87 and A172) and 3 types of human primary glioma cells (8591, LHH and 7112), demonstrating that LGR5 was expressed in the cell membrane and cytoplasm (Fig. 1a). This evidence concerns the gene LGR5 and central nervous system cancer.