Finally, we are aware that the current model represents a specific tubulointerstitial insult with no glomerular injury, thus not reflecting all types of CKD.More studies employing different timing and dose of fish oil treatment in this and other models of CKD are still warranted in order to better understand the link between Klotho reduction and inflammation, fibrosis, and renal dysfunction. The gene discussed is KL; the disease is chronic kidney disease.