In view of the presence of CKD [4,26,27], inflammation [15,16], or both, current experiments disclosed a marked reduction in renal Klotho expression in these adenine-fed animals, corroborating previously published data who showed extremely low levels of serum and renal Klotho after 4 and 6 weeks of adenine feeding [25,28]. The gene discussed is KL; the disease is chronic kidney disease.