When subjects were divided into 3 groups with respect to grade of steatosis, hepatic IGF-1 expression levels significantly decreased with advanced grade of steatosis (IGF-1/GAPDH cDNA ratio: steatosis grade 1 (n = 19): 2.72 ± 3.02; steatosis grade 2 (n = 5): 1.07 ± 1.52; steatosis grade 3 (n = 5): 0.56 ± 0.28, p = 0.01). This evidence concerns the gene GAPDH and steatosis.