The immunohistochemical evaluation for the presence of tumor-infiltrating T lymphocytes (CD4 and CD8) and macrophages (CD68) demonstrated a significantly higher prevalence of inflammatory infiltrate at the neoplastic invasive front of the primary tumor and of the ENE in contrast with what observed at the core of both CRCs and their matched intra-nodal metastases. Here, CD8A is linked to neoplasm.