ANO1 and intrahepatic cholangiocarcinoma: At day 7, ISMC Mix cultured on STO cells in F12 medium expressed the highest level of ICC markers (Kit and Ano1 co-localization), SMCs marker (MHC) and neuronal marker (β-tubulin), followed by ISMC Mix cultured on STO cells in FBS medium and ISMC Mix cultured on gelatin in FBS medium (Fig. 4a).