In the loss-of-function CRISPR/Cas9 screen, several genes with a known CRC regulatory role such as MYC, KLF5, CCND2, BPTF, TCF7L2, CTCF, and SMAD4 together with 58 other genes, were identified as required for cell growth in at least one of the three cell lines (Robust Rank Aggregation, FDR < 0.1). This evidence concerns the gene SMAD4 and colorectal carcinoma.