Although no changes in the biomarkers of oxidative stress were observed in animals with steatosis that had consumed tomato juice, the accumulation of lycopene appears to improve the levels of some amino acids related to antioxidant mechanisms and to modulate the expression of nuclear factors responsible for the regulation of lipid metabolism and cellular homeostasis, such as FXR and HNF4A. Therefore, based on the results obtained, tomatoes and tomato-derived products can be considered as part of a dietary strategy in the control and treatment of NAFLD, as natural and safe sources of lycopene. This evidence concerns the gene HNF4A and metabolic dysfunction-associated steatotic liver disease.