In our current study, we show that generally AA cell lines (E006AA, E006AA-hT, MDA-PCa-2b) display EMT markers Snail, vimentin, Twist, Cat L to levels similar to metastatic CA cell lines (DU145, PC3, C4-2, ARCaP), as well as lower E-cadherin as compared to normal (though immortalized) prostate epithelial RWPE-1, and non-metastatic LNCaP and 22Rv1 cell lines. The gene discussed is TWIST1; the disease is cancer.