The metastatic CA prostate cancer cell lines (DU145, PC3, ARCaP-E, ARCaP-M) as well as the tumorigenic but non-metastatic AA cell lines (E006AA, E006AA-hT, MDA-PCa -2b) expressed higher levels of Snail, vimentin, Cat L, Twist and lower levels of E-cadherin (with the exception of C4-2 and MDA-PCa-2b which still expressed high E-cadherin) (Fig 1B). Here, CTSL is linked to Familial prostate cancer.