Translating our TIC marker and Wnt pathway results from Apcmin mouse intestinal epithelia to human colon cancer cells, and consistent with CtBP’s regulation of TIC-enriched tumorspheres, LGR5 mRNA and/or protein expression was also effectively disrupted by CtBP2 knockout in HCT116 tumorspheres (Figure 5E), or by 4-Cl-HIPP treatment in HCT116 or HT29 tumorspheres (Figure 5F-5G). Here, CTBP2 is linked to malignant colon neoplasm.