In mice fed HFD, T-β-MCA is converted to MCA by bile salt hydrolase (BSH), a microbial enzyme, which results in aggravated hepatic steatosis and hepatic injury due to increased intestinal ceramide production via activation of TCA-mediated agonistic action for FXR and inhibition of T-β-MCA-mediated antagonistic action (85). The gene discussed is NR1H4; the disease is Hepatic steatosis.