Interestingly, the expression of all DE ncRNAs analyzed was modified by drug-induced inhibition of the MAPK pathway, whose hyperactivation is considered a key event for CRC onset and progression.46, 47 It is well known that the molecular effects of U0126 appear a few hours after treatment; accordingly, we observed a global downregulation all ncRNAs at 6 hr after treatment, except for HOTAIR and TUG1. The gene discussed is HOTAIR; the disease is colorectal carcinoma.