SMO and medulloblastoma: Furthermore, mutation of the putative miR-324-5p complementary seed sites within both the GLI1 and SMO 3′UTRs prevented miR-324-5p from exerting its repressive effects on the constructs (Fig. 1B and C), confirming miR-324-5p acts directly on the human SMO and GLI1 3′UTR, as proposed in medulloblastoma [27].