Cytotoxicity analysis by Annexin V/PI staining (Fig 2F) revealed that CG5 cells died for apoptosis around 36.6% of the population after treatment with PTX free and 28.4% after treatment with PTX-loaded ASC conditioned medium, indicating the high toxicity of conditioned medium comparable to free drug in CG5 cancer cells. The gene discussed is ANXA5; the disease is cancer.