Similar to other study, elevated ALT was used as a surrogate marker of NAFLD, and considered a common condition in subjects with T2DM.9 Other main finding of this study also shows the significant differences between the groups in age (if ALT>35), gender (more likely to be male) and triglyceride higher (if ALT>35) but not with HbA1c and obesity. This evidence concerns the gene GPT and obesity due to melanocortin 4 receptor deficiency.