Here we focused on exploring the potential to study neurodegenerative diseases by applying and optimizing the technique using transgenic zebrafish expressing mutated forms of the proteins fused in sarcoma (FUS) and ataxin-3 to model amyotrophic lateral sclerosis (ALS) and spinocerebellar ataxia type-3 (SCA3), respectively. The gene discussed is ATXN3; the disease is Spinocerebellar ataxia type 3.